Lack of Modifying Effects of Eugenol on Development of Lung Proliferative Lesions Induced by Urethane in Trans- Genic Mice Carrying the Human Prototype

ABSTRACT Tb investigate he modifying effects of eugenol (EUG), a component of cigarette smoke, on lung carcinogenesis, male and female transgenic mice carrying the human prototype c-Ha-sus gene (rasH2 mice) were given a single intraperitoneal i jection of 250 mgfkg urethane (UR) or saline, fo11owed by a diet containing 6,OOO ppm EUG or basal diet fbr 26 weeks, Their non-transgenic CB6Fl littermates (non-Tg mice) were also treated in the same rnanner. In both rnale and female rasH2 mice, alveolarfbronchiolar hyperplasias, adenomas and carcinomas were observed in all UR-treated groups. However, there were no significant intergroup differences in the incidences and multiplicities of these lesions between the UR alone and UR+EUG groups. In non-Tg mice, alveolathronchiolar hyperplasias, adenoTnas or carcinomas were sporadically observed in UR-treated groups of both sexes, with no significant differences in the incidences and multip]icities between the UR a]Qne and UR + EUG groups. There were no intergroup differences between them in the PCNA-positive ratios of adenomas or carcinomas and the areas of adenomas or carcinomas to the whole lung area examined, The present results suggest that the EUG treatment does not exert modifying effects on Iung carcinogenesis induced by UR in both male and female rasH2 mice and non-Tg mice,